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. 2014 May 15;2014:474953. doi: 10.1155/2014/474953

Figure 6.

Figure 6

Western blotting analysis for the protein expression of caspase 3 and cyclin D1 from human lung cancer cells A549 in the presence or absence of the methanolic fraction of S. grandiflora leaves. (a) Protein abundance of active caspase 3. (b) Protein abundance of cyclin D1. (c) Protein abundance of cytosolic NFκB. (d) Loading control β-actin. (e) Densitometry for the protein abundance of active caspase 3. (f) Densitometry for the protein abundance of cyclin D1. (g) Densitometry for the protein abundance of cytosolic NFκB. (h) Protein abundance of nuclear NFκB. (i) Loading control lamin B. (j) Densitometry for the protein abundance of nuclear NFκB. Values are mean ± S.D from three independent experiments. ANOVA followed by Tukey's test was used to assess the statistical significance between groups. *P ≤ 0.05, significance relative to control.