Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 17;16(2):R41. doi: 10.1186/bcr3645

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Garimella et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

PMC Copyright notice

A secondary screen of putative negative regulators of TRAIL-induced apoptosis. Four siRNAs corresponding to 16 genes were selected for additional analysis of TRAIL-induced caspase-3/7 activation in (A) MB231, (B) MB468, (C) T47D, and (D) SKBR3. Cells were siRNA transfected, as for the primary screen, and 48 hours later were treated with 1,000 ng/ml TRAIL for 1 hour before assessment of caspase-3/7 activation. The dashed line in each panel indicates the 2-standard deviation fold-increase in TRAIL-induced caspase-3/7 activation compared with TRAIL-treated cells transfected with negative control siRNA (siNeg). The vertical line joins the siRNAs for each gene. Genes are ranked in descending order based on the median value for each set of four siRNAs per gene in the MB231 cell line. Data for the control siRNAs, siNeg, siCASP8, and siFLIP are included for reference.