Table 1.
Sequences of the primers used to detect β-lactamases genes.
| PCR target | Primer name | Primer sequence | Annealing temperatures on °C | Amplicon sizes |
|---|---|---|---|---|
| CTX-M-1 group | CTX-1F | 5′ATGGTTAAAAAATCACTGCGTC3′ | 60 | 864 pb |
| CTX-1R | 5′TTGGTGACGATTTTAGCCGC3′ | 60 | ||
|
| ||||
| CTX-M-2 group | CTX-2F | 5′ATGATGACTCAGAGCATTCG3′ | 58 | 866 pb |
| CTX-2R | 5′TGGGTTACGATTTTCGCCGC3′ | 62 | ||
|
| ||||
| CTX-M-8 group | CTX-8F | 5′ACTTCAGCCACACGGATTCA3′ | 60 | 877 pb |
| CTX-8R | 5′CGAGTACGTCACGACGACTT3′ | 62 | ||
|
| ||||
| CTX-M-9 group | CTX-9F | 5′ATGGTGACAAAGAGAGTGCAA3′ | 60 | 876 pb |
| CTX-9R | 5′TCACAGCCCTTCGGCGATGATTCTCGC3′ | 86 | ||
|
| ||||
| SHV | SHV-1F | 5′ATGCGTTATATTCGCCTGTGTATT3′ | 66 | 868 pb |
| SHV-1R | 5′-TTAGCGTTGCCAGTGCTCGATCAG-3′ | 74 | ||
|
| ||||
| TEM | TEM-1F | 5′ATAAAATTCTTGAAGACGAAA3′ | 52 | 1080 pb |
| TEM-1R | 5′GACAGTTACCAATGCTTAATC3′ | 58 | ||