Figure 2. Free [Ca²⁺]_in profiles of PAO1 WT (black line) and efhP mutant strain PAO1043 (grey line).

Cultures were grown in 0²⁺. After the basal level of [Ca²⁺]_in was monitored for 1 min, 1 mM Ca²⁺ was added (indicated by the arrow) followed by further [Ca²⁺]_in measurement for 20 min.