. 2014 Jun 11;9(6):e98985. doi: 10.1371/journal.pone.0098985

Table 1. Proteins identified by MASCOT-based analysis of MALDI-TOF generated mass spectra.

Protein name	Genename	PAno.	Genetic context	^aInduced in biofilm, or byCa²⁺ in PAO1 or FRD1	Normalized signal intensity (NV)^b at 10 mM Ca²⁺
					PAO1		PAO1043		FRD1		FRD1043
					P^c	B^d	P	B	P	B	P	B
Iron acquiring and storage
Pyoverdine biosynthesis protein PvdN	pvdN	2394	pvd operon	BiofilmCa²⁺	N^e	0.22	N	N	0.05	0.02	N	N
Pyoverdine biosynthesis protein PvdO	pvdO	2395	pvd operon	BiofilCa²⁺	N	0.13	N	N	0.15	0.09	N	N
Fe(III)-pyochelin receptor precursor FptA	fptA	4221	fptAB and pchEFR operons	BiofilmCa²⁺	N	0.19	N	N	0.17	0.09	N	0.03
Ferric iron-binding periplasmic protein HitA	hitA	4687	hitB	BiofilmCa²⁺	0.16	0.19	0.19	0.15	0.19	0.12	N	N
Probable binding protein component of ABC Fe transporter		5217	ABC iron transport	Ca²⁺	0.11	0.08	0.09	0.07	0.05	0.02	N	N
Stress response
Heat shock protein HtpG	htpG	1596	Hypothetical	BiofilmCa²⁺	0.51	0.24	0.24	0.16	0.38	0.31	0.24	N
^fPredicted universal stress protein UspA		4352	Hypothetical	Biofilm	0.05	0.18	0.12	0.15	N	0.20	0.03	0.04
Hypothetical		1069	Heat shock Hsp90	^gBiofilm Ca²⁺	0.05	0.01	N	N	0.03	0.13	N	N
Phosphoenolpyruvate carboxykinase	pckA	5192	Heat shock Hsp33	^gCa²⁺	0.28	0.2	0.24	0.04	0.39	0.3	0.15	N
Protein degradation
Protease PfpI	pfpI	0355	Hypothetical	BiofilmCa²⁺	0.09	0.14	N	0.04	0.20	0.06	N	N
Protease IV	Piv	4171			N	N	N	N	0.12	0.09	N	N
Trigger factor	Tig	1800	clpPX proteases	BiofilmCa²⁺	0.43	0.34	0.41	0.08	0.19	0.59	0.19	0.11
Phenazine biosynthesis
Phenazine biosynthesis protein PhzB1	phzB1	4211	phz operon	BiofilmCa²⁺	N	0.09	N	N	N	0.19	N	N
Phenazine biosynthesis protein PhzD1	phzD1	4213	phz, operon	BiofilmCa²⁺	0.02	0.20	N	0.03	N	0.28	N	0.05
Phenazine biosynthesis protein PhzB2	phzB2	1900	phz, operon	BiofilmCa²⁺	N	0.61	N	N	0.22	1.15	N	N

Based on the data in [69]. ^bAverage normalized volumes over at least two biological replicates under each of the tested growth conditions : ^cP – planktonic, ^dB – biofilm. ^eN - not detected. For protein identification, the MASCOT scores were greater than 75 (p<0.05), and the minimum number of peptides matched was 5. ^fProtein name was changed based on sequence analysis. ^gBased on unpublished data. Significant changes in the mutants are shown in bold.