Table 1. Proteins identified by MASCOT-based analysis of MALDI-TOF generated mass spectra.
Protein name | Genename | PAno. | Genetic context | aInduced in biofilm, or byCa2+ in PAO1 or FRD1 | Normalized signal intensity (NV)b at 10 mM Ca2+ | |||||||
PAO1 | PAO1043 | FRD1 | FRD1043 | |||||||||
Pc | Bd | P | B | P | B | P | B | |||||
Iron acquiring and storage | ||||||||||||
Pyoverdine biosynthesis protein PvdN | pvdN | 2394 | pvd operon | BiofilmCa2+ | Ne | 0.22 | N | N | 0.05 | 0.02 | N | N |
Pyoverdine biosynthesis protein PvdO | pvdO | 2395 | pvd operon | BiofilCa2+ | N | 0.13 | N | N | 0.15 | 0.09 | N | N |
Fe(III)-pyochelin receptor precursor FptA | fptA | 4221 | fptAB and pchEFR operons | BiofilmCa2+ | N | 0.19 | N | N | 0.17 | 0.09 | N | 0.03 |
Ferric iron-binding periplasmic protein HitA | hitA | 4687 | hitB | BiofilmCa2+ | 0.16 | 0.19 | 0.19 | 0.15 | 0.19 | 0.12 | N | N |
Probable binding protein component of ABC Fe transporter | 5217 | ABC iron transport | Ca2+ | 0.11 | 0.08 | 0.09 | 0.07 | 0.05 | 0.02 | N | N | |
Stress response | ||||||||||||
Heat shock protein HtpG | htpG | 1596 | Hypothetical | BiofilmCa2+ | 0.51 | 0.24 | 0.24 | 0.16 | 0.38 | 0.31 | 0.24 | N |
fPredicted universal stress protein UspA | 4352 | Hypothetical | Biofilm | 0.05 | 0.18 | 0.12 | 0.15 | N | 0.20 | 0.03 | 0.04 | |
Hypothetical | 1069 | Heat shock Hsp90 | gBiofilm Ca2+ | 0.05 | 0.01 | N | N | 0.03 | 0.13 | N | N | |
Phosphoenolpyruvate carboxykinase | pckA | 5192 | Heat shock Hsp33 | gCa2+ | 0.28 | 0.2 | 0.24 | 0.04 | 0.39 | 0.3 | 0.15 | N |
Protein degradation | ||||||||||||
Protease PfpI | pfpI | 0355 | Hypothetical | BiofilmCa2+ | 0.09 | 0.14 | N | 0.04 | 0.20 | 0.06 | N | N |
Protease IV | Piv | 4171 | N | N | N | N | 0.12 | 0.09 | N | N | ||
Trigger factor | Tig | 1800 | clpPX proteases | BiofilmCa2+ | 0.43 | 0.34 | 0.41 | 0.08 | 0.19 | 0.59 | 0.19 | 0.11 |
Phenazine biosynthesis | ||||||||||||
Phenazine biosynthesis protein PhzB1 | phzB1 | 4211 | phz operon | BiofilmCa2+ | N | 0.09 | N | N | N | 0.19 | N | N |
Phenazine biosynthesis protein PhzD1 | phzD1 | 4213 | phz, operon | BiofilmCa2+ | 0.02 | 0.20 | N | 0.03 | N | 0.28 | N | 0.05 |
Phenazine biosynthesis protein PhzB2 | phzB2 | 1900 | phz, operon | BiofilmCa2+ | N | 0.61 | N | N | 0.22 | 1.15 | N | N |
Based on the data in [69]. bAverage normalized volumes over at least two biological replicates under each of the tested growth conditions : cP – planktonic, dB – biofilm. eN - not detected. For protein identification, the MASCOT scores were greater than 75 (p<0.05), and the minimum number of peptides matched was 5. fProtein name was changed based on sequence analysis. gBased on unpublished data. Significant changes in the mutants are shown in bold.