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. 2014 Jun 11;9(6):e97125. doi: 10.1371/journal.pone.0097125

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© 2014 Leng et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Hepatic DDAH1 & DDAH2 mRNA levels (panel A) were detected by RT-PCR, and protein expressions of DDAH1 & DDAH2 (panel B) were detected by Western blotting. The graphs in panel B represent the quantification of relative DDAH1 or DDAH2 vs β-actin protein levels. In addition, activities of DDAH (panel C) and NOS (panel E), contents of ADMA in serum (panel D) and nitrite/nitrate in the liver (panel F) were measured in control and diabetic rats. Data are expressed as mean ± SEM, n = 5, *P<0.05, **P<0.01 vs Control.