Figure 8. Effects of PDTC on the enhance of oxidative stress and suppression of nitric oxide production in rat hepatocytes induced by ADMA.

Rat hepatocytes (H4IIE cell line) were untreated (Control) or treated with 0.5 mM hydrogen peroxide (H₂O₂) for 1 h, 30 µM asymmetric dimethylarginine (ADMA), 30 µM N^G-Nitro-L-arginine Methyl Ester (L-NAME) and 10 µM Pyrrolidine dithiocarbamate (PDTC) for 48 h, or preincubated with 10 µM PDTC for 2 h and then following the co-incubation with 0.5 mM H₂O₂ (PDTC+H₂O₂) for 1 h, 30 µM ADMA (PDTC+ADMA), 30 µM L-NAME (PDTC+L-NAME) for 48 h. Oxidative stress was reflected by the content of lipid peroxidation production malondialdehyde (MDA, panel A) in the cell conditioned media and the activity of antioxidant enzyme superoxide dismutase (SOD, panel B) in hepatocytes. Nitric oxide (NO) production was evaluated by the activity of nitric oxide synthase (NOS, panel C) in hepatocytes and the content of NO metabolites nitrate/nitrite (panel D) in the cell conditioned media. Data are expressed as mean ± SEM, *P<0.05, **P<0.01 vs Control; ⁺ P<0.05, ⁺⁺ P<0.01 vs H2O2; ^## P<0.01 vs ADMA; ^$ P<0.05, ^$$P<0.01 vs L-NAME.