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. 2014 Jun 11;9(6):e97125. doi: 10.1371/journal.pone.0097125

Table 2. Reaction condition of polymerase chain reaction.

Genes Initial denature Cycle parameters Final extension Cycle number
Bip 94°C 5 min 94°C 30 s, 53°C 30 s, 72°C 30 s; 72°C 10 min 30
XBP-1 94°C 5 min 90°C 60 s, 60°C 60s, 70°C 60 s, 72°C 10 min 30
CHOP 94°C 5 min 94°C 30 s, 52.2°C 30 s, 72°C 30 s, 72°C 10 min 30
DDAH1 94°C 5 min 94°C 45 s, 55°C 45 s, 72°C 45 s, 72°C 10 min 30
DDAH2 94°C 5 min 94°C 45 s, 64°C 45 s, 72°C 45 s, 72°C 10 min 30