Table 2. Reaction condition of polymerase chain reaction.
Genes | Initial denature | Cycle parameters | Final extension | Cycle number |
Bip | 94°C 5 min | 94°C 30 s, 53°C 30 s, 72°C 30 s; | 72°C 10 min | 30 |
XBP-1 | 94°C 5 min | 90°C 60 s, 60°C 60s, 70°C 60 s, | 72°C 10 min | 30 |
CHOP | 94°C 5 min | 94°C 30 s, 52.2°C 30 s, 72°C 30 s, | 72°C 10 min | 30 |
DDAH1 | 94°C 5 min | 94°C 45 s, 55°C 45 s, 72°C 45 s, | 72°C 10 min | 30 |
DDAH2 | 94°C 5 min | 94°C 45 s, 64°C 45 s, 72°C 45 s, | 72°C 10 min | 30 |