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. 2014 Jun 11;9(6):e99214. doi: 10.1371/journal.pone.0099214

Figure 1. Rb represses HIF1-regulated target gene mRNA in MCF7 human breast cancer cells and LNCaP human prostate cancer cells.

Figure 1

MCF7 cells (A, B, C and D) and LNCaP cells (E) were transfected with either scrambled siRNA (SCX) or Rb siRNAs siRb 1, and siRb 2. Twenty-four hours after transfection cells were either maintained under normoxic conditions or 1% O2 for a further 24 h. Gene expression was determined by quantitative real-time PCR after isolation and reverse transcription of total RNA. VEGF, CXCR4, PLOD2 and Rb expression were normalized to constitutively active 36B4 gene expression. Open bars represent normoxia (20% O2) and closed (grey) bars represent hypoxia (1% O2). Error bars represent ± S.D. *p<0.05.