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. 2014 Jun 11;9(6):e99214. doi: 10.1371/journal.pone.0099214

Figure 4. Rb represses HIF1-regulated target gene protein accumulation in MCF7 human breast cancer cells and is phosphorylated at serines 780 and 807/811.

Figure 4

MCF7 cells (A) were transfected with either scrambled siRNA (SCX) or Rb siRNAs siRb 1, and siRb 2. Rb, CXCR4, HIF1α, PLOD2 and α-tubulin protein levels were assessed by immuno-blot after exposure to atmospheric O2 or 1% O2 for 48 (Rb and CXCR4) or 96 h (HIF1α, and PLOD2). (B) Immuno-blots of whole cell lysates from MCF7 and LNCaP cells either left at normoxia (N) or treated with 1% O2 for 6 h (H). Blots were probed with primary antibodies to total Rb, Rb-phospho-serine780 (Rb-pS780), Rb-phospho-serine807/811 (Rb-pS807/811), or α-tubulin as a loading control.