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. 2014 Jun 11;9(6):e99610. doi: 10.1371/journal.pone.0099610

Figure 3. RIG-I-enhanced type I IFN-independent apoptosis in response to JUNV infection.

Figure 3

A–B. shRNA-mediated stable down-regulation of RIG-I expression diminished DNA fragmentation in infected A549 cells. A. Efficient knock-down of RIG-I expression in RIG-I KD cells. Following poly(I:C) transfection RIG-I deficient (RIG-I KD) or competent (Control KD) cells were lysed and subjected to western blotting analysis. B–D. Cells were infected (MOI = 1.75 PFU/cell) with Candid#1 or Romero JUNV or mock-infected (Mock). B. Diminished DNA fragmentation in infected RIG-I-KD versus that of Control KD cells. C. Diminished DNA fragmentation in infected RIG-I-deficient Huh7.5 cells versus that of RIG-I competent Huh7. D. Induction of DNA fragmentation in type I IFN-deficient Vero and Vero E6 cells upon Candid#1 or Romero infection, respectively. B–C. Accumulation of mono- and oligonucleosomes in the cytoplasm was assayed by ELISA. Data represent the average of 3 replicates ±SEM. *** - P. value<0.001.