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. 2014 Jun 11;9(6):e99026. doi: 10.1371/journal.pone.0099026

Figure 4. LC-CID fragmentation of IgA1 Ser232-Arg245 (HR) with GalNAc1Gal1NeuAc1.

Figure 4

(A) Ser232-Arg245 HR O-glycan profile of IgA1 (Ale) myeloma protein enzymatically sialylated with ST3Gal1. (B) LC-CID tandem MS spectrum of Ser232-Arg245 with GalNAc1Gal1NeuAc1. Absence of sialylated GalNAc (shown in blue parenthesis) indicates that NeuAc is attached to Gal (by an α2,3-linkage) in the precursor ion. Additionally, the presence of the Gal-NeuAc oxonium ion confirms the attachment of the NeuAc to Gal.