Figure 5. Treatment with Anakinra reduced neutrophil recruitment to the airways at the peak of viral-induced inflammation.

(A) BALB/c mice received Anakinra or PBS twice every day, starting two days prior to the viral infection and until mice were sacrificed. (B) Viral load was determined by quantitative real-time PCR and normalized to GAPDH. (C) Proportion of neutrophils recruited to the airways following influenza infection (day 1–5) or PBS challenge (day 0) was assessed by flow cytometry. Two representative FACS plots of each group at day 5 after infection are shown as well as the plotted data. FACS plots show Ly-6C versus Ly-6G expression of CD11c⁻ CD11b⁺ pre-gated cells and indicate the frequency of neutrophils of all live cells. Data are representative of two independent experiments (n = 4–5), error bars indicate s.e.m; i.n. (intranasal); p.i. (post infection); ctrl (control).