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. 2014 Jun 11;9(6):e99234. doi: 10.1371/journal.pone.0099234

Figure 3. Gene expression of Fe deficiency and SA response genes in 3xbhlh and wild type plants in response to SA and Fe.

Figure 3

A, BHLH38, OBP3, PR1; B, FIT, FRO2, IRT1; 3xbhlh and wild type seedlings were grown for 6 d at + and −Fe and exposed for 6 h to 100 µM SA (+SA) or were mock-treated (−SA). Whole seedlings were harvested for analysis. n = 2; * indicates a significant change (p<0.05) of −Fe versus +Fe; + indicates a significant change (p<0.05) of 3xbhlh versus WT; § indicates a significant change (p<0.05) of +SA versus –SA. Gene expression was studied using reverse transcription-qPCR.