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. 2014 Jun 11;9(6):e98636. doi: 10.1371/journal.pone.0098636

Figure 5. Effect of LPS on inflammatory cytokines in PBMCs.

Figure 5

SIVmac239-infected animals were intravenously injected with a single dose of LPS (50 µg/kg, solid circles and lines, n = 3) or saline (open circle and dashed lines, n = 3) at 45 weeks postinfection. Blood samples were collected at indicated time points post-LPS administration and PBMCs were isolated. The levels of cytokines (IL-6, IL-8, IFN-α, and TNF-α) in PBMCs were determined by real-time PCR and normalized to GAPDH mRNA. Data are expressed as fold of control (before LPS administration, cytokine mRNA/GAPDH mRNA, which was defined as 1).