Table 2. Primer Pairs for Real-Time RT-PCR.
Primer | Orientation | Sequences (5′→3′) |
SIV gag | Forward | GCAGAGGAGGAAATTACCCAGTAC |
Reverse | CAATTTTACCCAGGCATTTAATGTT | |
IL-6 | Forward | TGGCTGAAAAAGATGGATGCT |
Reverse | TTGCTCCTCACTACTCTCAAACCT | |
IL-8 | Forward | ACTGAGAGTGATTGAGAGTGGAC |
Reverse | AACCCTCTGCACCCATGGTTC | |
IFN-α | Forward | GCCTGAAGGACAGACATGACTTT |
Reverse | GGATGGTTTGAGCCTTTTGG | |
TNF-α | Forward | GGCTCAGGCAGTCAGATCATC |
Reverse | GCTTGAGGGTTTGCTACAACATG | |
GAPDH | Forward | GTCTGGAAAAACCTGCCAAG |
Reverse | ACCTGGTGCTCAGTGTAGCC |