Figure 2. Plasmid construction and transfection.

(a) The confirmatory sequencing of the insert in the plasmid. (b) The pGCMV/EGFP overexpression vector kit. (c) The transfection efficiency of three groups was detected by fluorescence microscopey (×200). This picture depicts the blank group without the green fluorescent signal. The control group and the experimental group with have bright green fluorescence signals. (d) The fluorescence photograph of LoVo cells which are stably transfected with the negative control plasmid and the AZGP1 overexpression plasmid (×200 magnification).