Skip to main content
. 2014 Jun 11;9(6):e100033. doi: 10.1371/journal.pone.0100033

Figure 3. The UPRer but not the UPRmt is activated in the hmgr-1(tm4368) mutant.

Figure 3

(A–B) The UPRer reporter Phsp-4::GFP is expressed in wild-type L4 worms exposed to 1 mM fluvastatin for 24 hours. (C–D) Phsp-4::GFP is also expressed in hmgr-1(tm4368) mutant L4s deprived of mevalonate for 24 hours, but is silenced within 72 hours when these worms are subsequently provided 20 mM mevalonate. (G–J) hmgr-1(4368) mutants frequently expressed high levels of Phsp-4::GFP in what appear to be disorganized embryos on either side of the spermatheca (two are indicated by arrowheads in H). (K–L) The UPRmt reporters Phsp-6::GFP and Phsp-60::GFP are not expressed in the hmgr-1(tm4368) deprived of mevalonate for 24 hours.