Table 3.
Mitochondrial oxidative stress-related measurements
| Control (sham operated) | Ischemia/Reperfusion | |||||||
|---|---|---|---|---|---|---|---|---|
| Mclk1+/+ | Mclk1+/− | Mclk1+/+ | Mclk1+/− | |||||
| Young | Old | Young | Old | Young | Old | Young | Old | |
|
| ||||||||
| Mitochondrial Aconitase activity (nmol/mg/min) | 4.7a ± 0.6 | 7.5 ± 1.1 | 4.8 ± 0.6 | 8.4 ± 1.1 | 3.1 ± 0.6 | 3.6 ± 0.7 | 5.6 ±0.7* | 2.5 ± 0.5 |
|
| ||||||||
| Cytosolic Aconitase activity (nmol/mg/min) | 19.3 ± 2.1 | 16.1 ± 0.9 | 22.1 ± 2.5 | 15.7 ± 1 | 14.9 ±1.3 | 14.6 ±1.1 | 19.2±1.5* | 15.4 ±0.8 |
|
| ||||||||
| Mitochondrial α-KGDH activity (nmol/mg/min) | 19.6 ± 1.4 | 18.1 ± 2 | 21.5 ± 1.9 | 19.4 ± 1.6 | 17.3 ±1.6 | 15.2 ± 1.9 | 23.4±1.4* | 16.4 ± 2 |
|
| ||||||||
| Whole brain TBARS (nmol/g) | 2.7 ± 0.3 | n.d. | 2.4 ± 0.3 | n.d. | 4.4 ± 0.5 | n.d. | 3.2± 0.2* | n.d. |
a
data are means ± SEM of 10–18 samples
*
significantly different from the corresponding controls (Mclk1+/+) at P < 0.05