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. 2014 Jun 11;9(6):e99371. doi: 10.1371/journal.pone.0099371

Figure 1. Down-regulation of BRCA1 in developing mouse retina implicates the attenuation of DNA repair.

Figure 1

A, The protein expression level of BRCA1 was developmentally down-regulated in the mouse retina. β-actin was included as a loading control. B, Relative quantification of the expression of BRCA1 in the retina was quantified by densitometry. BRCA1 in retina significantly and gradually decreased with the age of the animal. C, DNA end joining efficiency was detected by rejoining the linearized plasmid [pcDNA3–1-His] with 5′ overhang [EcoRI] and blunt [EcoRV] ends in the presence of the nuclear protein extracts from retinal neurocytes. A negative control sample with cell-free extracts was analyzed in parallel. Gels are representative of three independent experiments. D, Relative quantification of DNA NHEJ of retinal neurocytes. The efficiency of DNA repair of retinal neurocytes at one month is significantly lower than that of postnatal day 1. All results were confirmed in three independent experiments. Error bars represent the standard deviation of the mean [n = 3]. Asterisks indicate statistically significant differences between the control and test cells [*p<0.05].