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. 2014 Jun 11;9(6):e99358. doi: 10.1371/journal.pone.0099358

Table 1. Panels of neutral loci and immune-related loci used to screen samples of Atlantic salmon, Salmo salar, from the salmon industry in Chile.

Locus Type Motifa Rangeb TA c Labeld Primerse Referencef
Neutral
SSsp1605 (GATA)11 214–252 58 Fam F: CGCAATGGAAGTCAGTGGACTGGR: CTGATTTAGCTTTTTAGTGCCCAATGC [50]
SSsp2201 (GATA)34 251–335 58 Fam F: TTTAGATGGTGGGATACTGGGAGGCR: CGGGAGCCCCATAACCCTACTAATAAC [50]
SSsp2210 (GTTA)11 112–168 58 Fam F: AAGTATTCATGCACACACATTCACTGCR: CAAGACCCTTTTTCCAATGGGATTC [50]
SSsp2213 (GTTA)22 151–215 58 Hex F: ATGTGGAGGTCAACTAACCAGCGTGR: CATCAATCACAGAGTGAGGCACTCG [50]
SSsp2215 (GTTA)14 107–175 58 Ned F: ACTAGCCAGGTGTCCTGCCGGTCR: AGGGTCAGTCAGTCACACCATGCAC [50]
SSsp2216 (GTTA)25 201–257 58 Ned F: GGCCCAGACAGATAAACAAACACGCR: GCCAACAGCAGCATCTACACCCAG [50]
SSspG7 (GTTA)18 121–215 58 Hex F: CTTGGTCCCGTTCTTACGACAACCR: TGCACGCTGCTTGGTCCTTG [50]
SSa85 (GT)14 106–186 58 Ned F: AGGTGGGTCCTCCAAGCTACR: ACCCGCTCCTCACTTAATC [51]
SSa197 (GT)5C(TG)4TC(TG)3A(GTGA)15 162–260 58 Fam F: GGGTTGAGTAGGGAGGCTTGR: TGGCAGGGATTTGACATAAC [51]
SSa171 (TGTA)14(TG)7 198–252 58 Fam F: TTATTATCCAAAGGGGTCAAAAR: GAGGTCGCTGGGGTTTACTAT [51]
SSa202 (CA)3(CTCA)17 237–371 58 Ned F: CTTGGAATATCTAGAATATGGCR: TTCATGTGTTAATGTTGCGTG [51]
SSa14 (TC)10N15(TC)3N2(AC)12(TC)3N5(CA)4 161–165 NA Tail F: CCTTTTGACAGATTTAGGATTTCR: CAAACCAAACATACCTAAAGCC [52]
SSOSL85 (GT)22 201–241 NA Tail F: TGTGGATTTTTGTATTATGTTAR: ATACATTTCCTCCTCATTCAGT [53]
SSOSL311 (TG)38 139–190 NA Tail F: TAGATAATGGAGGAACTGCATTCTR: CATGCTTCATAAGAAAAAGATTGT [53]
SSOSL438 (AC)26AT(AC)8 132–174 NA Tail F: GACAACACACAACCAAGGCACR: TTATGCTAGGTCTTTATGCATTGT [53]
Locus Type Motif a Range b TA c Label d Primers e Reference f
Immune-related
SsaIR001TKU (AG)12 113–115 NA Tail F: AAGAGCGAGAGAGAAGGATGGR: GTTTCACAGAATCAACAGTCAGCAA [47]
SsaIR002TKU (AG)10 241–249 NA Tail F: GGGTACAAGCAGGGGTCTTAR: GTTTAAGAGTGGACCGACAACAAT [47]
SsaIR003TKU (GT)13 146–163 NA Tail F: TGTTCTGCAGGTCAGAAGTGAR: GTTTGAGTGGGAGGAAGGGGAGTA [47]
SsaIR004TKU (AG)10 313–321 NA Tail F: AGCTATTTCCAAGGCGTTCAR: GTTTCACCACTCAGGAGAGCATGA [47]
SsaIR005TKU (CTT)7 371–379 NA Tail F: CGACGACTTTTTCATCTGTCTTR: GTTTGGACAACACATCTCATTCCAA [47]
SsaIR007TKU (AG)9 183–191 NA Tail F: GCAATGCTGCCATCTAGTGAR: GTTTCAAGGAAAGCCTACAAAAAGC [47]
SsaIR010TKU (AG)10 192–198 NA Tail F: CAACGACACCATACCAACCAR: GTTTAACCCCTTCCAAGTTCCATC [47]
SsaIR011TKU (ATC)8 364–376 NA Tail F: CCAGCCAACTACGACAACTGR: GTTTGTGGTTATTTTTGGGGTGA [47]
SsaIR012TKU (AATC)14 162–222 NA Tail F: GAGTCCCCTTTGGCCTCTCR: GTTTAAACACAGTAAGCCCATCTATTG [47]
SsaIR014TKU (ATC)6 395–407 NA Tail F: CTGAGGTGGTGGCACAGCR: GTTTATTGTTTGGTTCTTACAGCAGGA [47]
SsaIR016TKU (AT)9 317–327 NA Tail F: CCAAAAATGTCCCATTCACCR: GTTTGTGTGCCACTCAGAATTG [47]
BG934281 (TCTG)14 215–277 NA Tail F: ACTGCTTCTCCCCTGCTACAR: GTTTGCGAACCACACATATACCAC [46]
CA769358 (AC)22 133–173 NA Tail F: TGACGCCATATGCAAAGAGAR: GTTTCCTTTGTCTGCAAAACGTGA [46]
UBA (CA)17 330–364± NA Tail F: GGAGAGCTGCCCAGATGACTTR: CAATTACCACAAGCCCGCTC [44]
MHC_II GTTATTAAAT 232–292 NA Tail F: GATGGCAAAGAGGAAAGTGAGR: TTGTTATGCTCTACCTCTGAA [45]
a

Motif indicates repeat motif.

b

Range refers to alleles thus far uncovered (may include the 21 bp 50-tail-sequence).

c

TA is optimized annealing temperature. For loci amplified with the tail protocol, a step-down protocol was used so annealing temperature is not applicable (NA).

d

Label refers to dye (6-Fam, Ned, or Hex) used to label forward primer. Tail refers to loci that were amplified using the tail protocol.

e

Primer sequences are forward (F and reverse (R).

f

Citations describing putative function.