Figure 2. Association between maternal smoking and AHRR methylation in CBMCs. Maternal smoking throughout pregnancy is associated with lower methylation at (A) CpG_A7, (B) across assay A and (C) across assay B. Maternal smoking early in pregnancy was not associated with lower AHRR methylation. (D) 8 CpG sites, contained within 6 CpG units, show lower methylation (dB > 0.05, P < 0.05) in response to maternal smoking. The difference in methylation is limited to the CpG Island shore, whereas the CGI is hypomethylated in all CBMC samples. Y-axis = DNA methylation level. Grey shaded box = “cg05575921” CpG site of interest. Error bars = 95% CI.