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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2014 Mar 12;72:55–65. doi: 10.1016/j.freeradbiomed.2014.03.001

Figure 4. Western blot analysis of the identified HNE adducted proteins.

Figure 4

(A) 30 µg of total mouse heart homogenate and 10 µg of mouse mitochondrial homogenate from 3-days DOX-treated mice were loaded in gels and blotted with different antibodies as indicated. β-Tubulin and SOD2 were used as total heart homogenate and mitochondrial fraction loading controls, respectively. (B) Total H9C2 cell extracts treated with indicated concentration of DOX for 3 days were loaded and blotted with indicated antibodies. The experiments were repeated 4 times.