Table 1.

Mouse mitochondrial cardiac proteins showing increased HNE levels at 3 days after DOX injection.

Protein Identified	Accession #	Coverage	Number of peptidesa	Score	MW (kDa)	pI	t-testc	Foldb change
ATP synthase subunit beta	P56480	17.6	6	31.0	56.3	5.34	0.036	10.9
Dihydrolipoyl dehydrogenase	O08749	22.2	9	57.8	54.2	7.90	0.054	8.94
Succinate dehydrogenase [ubiquinone] flavoprotein subunit	Q8K2B3	15.7	6	42.3	72.5	7.37	0.057	7.20
Trifunctional enzyme subunit alpha	Q8BMS1	24.0	16	137	82.6	9.14	0.038	4.97
Creatine kinase S-type	Q6P8J7	41.0	13	118	47.4	8.40	0.051	4.40
Isoform Cytoplasmic of Fumarate hydratase	P97807-2	12.4	4	27.1	50.0	7.94	0.029	3.66
Succinyl-CoA:3-ketoacid-coenzyme A transferase 1	Q3UJQ9	15.8	4	12.5	52.2	8.94	0.057	3.46
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2	Q91WD5	48.0	16	145	52.6	6.99	0.058	1.98

^aThe number of peptide sequences identified by nanospray ESI-MS/MS of tryptic peptides.

^bThe fold-change in spot density from DOX treated compared with Saline treated.

^CThe t value associated with fold-change calculated using a Student’s t test.