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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Surg Res. 2014 Mar 22;190(1):1–8. doi: 10.1016/j.jss.2014.03.051

Figure 2.

Figure 2

Receptor levels of TLR are similar between VICs from the four heart valves. Untreated cell lysates were analyzed for TLR-4 using immunoblotting (A). TLR-4 levels did not differ significantly between VICs from different valves. Cells were treated with LPS (100ng/mL) for 24 hours and lysates were analyzed for TLR-4 levels. Shown above is the aortic valve data normalized to control (B). There was no significant change between control and LPS-stimulated levels of the TLR-4. This applied to the other three valves as well (data not shown).