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The gene sequences for dandelion PPOs 1, 2, 6 and 7 (excluding the transit peptide) were each supplemented with an N-terminal Strep2 tag using the enterokinase recognition site as a spacer. Proteins were purified by StrepTactin affinity chromatography and 3-µg samples were analyzed by SDS-PAGE using a non-reducing loading dye, followed by staining with Coomassie Brilliant Blue.
