Skip to main content
. 2014 Jun 11;9(6):e99514. doi: 10.1371/journal.pone.0099514

Figure 4. Zebrafish Rspo3 regulates dorsoventral patterning.

Figure 4

(A) Expression patterns of the indicated marker genes in wild-type (WT) embryos or embryos injected with 4 ng control MO (cMO), 4 ng rspo3 targeting MO1 or 600 pg rspo3 mRNA, respectively. Embryos were analyzed at the shield stage by in situ hybridization using the indicated probes. Panels a–d and i-p are top views with animal pole up and dorsal to the right; panels e–h are lateral and dorsal views with animal pole up. Arrows indicate the width of the expression domains; asterisks indicate the edges of the expression domains. Percentages of embryos in each category were calculated and are shown in B (chd), C (gsc), D (eve1) and E (ved). The total embryo numbers from three independent experiments are shown on the top of each bar. Scale bar = 200 µm.