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. 2014 Jun 11;9(6):e99514. doi: 10.1371/journal.pone.0099514

Figure 5. Rspo3 does not promote maternal Wnt/β-catenin signaling in zebrafish embryos.

Figure 5

(A, B) Effect of rspo3 knockdown (A) and overexpression (B) on the expression of boz mRNA. (C, D) Effect of rspo3 knockdown (C) and overexpression (D) on the expression of chd mRNA. One-cell stage embryos were injected with 8 ng control MO (cMO), 4 ng MO1 and 8 ng MO2, respectively (A, C), or 600 pg gfp mRNA or rspo3 mRNA (B, D). Injected embryos were analyzed by RT-qPCR at the sphere stage. Values are means ± S.E. (n = 3). *P<0.05; n.s., not significant, unpaired t-Test.