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. 2014 Jun 11;9(6):e99514. doi: 10.1371/journal.pone.0099514

Figure 7. Effects of rspo3 knockdown and forced expression on the expression of fgf3, fgf8, mkp3, and sqt mRNA.

Figure 7

One-cell stage embryos were injected with cMO (8 ng), MO1 (4 ng), MO2 (8 ng), gfp mRNA (600 pg), or rspo3 mRNA (600 pg), respectively. Injected embryos were raised to the 90% epiboly stage. The mRNA levels of sqt, fgf3, fgf8, and mkp3 were measured by RT-qPCR, normalized by β-actin mRNA levels, and as shown. Values are means ± S.E. (n = 3). *, **P<0.05 and 0.01 vs. the gfp or cMO group.