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. 2014 Jun 11;9(6):e99514. doi: 10.1371/journal.pone.0099514

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© 2014 Rong et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The phenotypes of embryos injected with 600 pg gfp or RSPO3 mRNA were scored and presented following the criteria described in Fig. 3A. The results are from three independent experiments and the total embryo numbers are given at the top. (B–F) Human RSPO3 alters the expression of the indicated genes in zebrafish embryos. Embryos injected with 600 pg RSPO3 or gfp mRNA were analyzed by whole mount in situ hybridization at the shield stage using the indicated probes. Scale bar = 200 µm. Percentages of embryos in each category were calculated and shown in C (chd), D (gsc), E (eve1), and F (ved). The total embryo numbers from three independent experiments are shown on the top of each bar.