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. Author manuscript; available in PMC: 2014 Jun 12.
Published in final edited form as: J Immunol. 2010 Oct 15;185(10):5943–5952. doi: 10.4049/jimmunol.0902795

FIGURE 1.

FIGURE 1

OVA uptake by macrophage cell lines treated with EdTx derivatives. Mouse macrophage cell lines RAW264.7 and J774A.1 were plated on a 24-well plate at 106 cells/ml and treated for 24 h with the following combination of EdTx subunits (2 µg/ml): 1) PAwt (PA) alone; 2) EF-S414N alone; 3) EF; 4) PA + EF-S414N; 5) PA + EF; 6) PA-U7 + EF-S414N; 7) PA-dFF + EF-S414N. Medium was removed and cells were incubated for 15 min with OVA-Alexa Fluor 488 (5 µg/ml; Molecular Probes). The uptake of OVA was assessed by flow cytometry and expressed as percentage of GFP-positive cells (n = 3 separate experiments). Results are expressed as mean ± 1 SD. Statistical significance: *p ≤ 0.05 when compared with control-untreated cells.