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. Author manuscript; available in PMC: 2014 Jun 12.
Published in final edited form as: J Immunol. 2010 Oct 15;185(10):5943–5952. doi: 10.4049/jimmunol.0902795

FIGURE 2.

FIGURE 2

Costimulatory molecule expression in macrophage cell lines treated with EdTx derivatives. Mouse macrophage cell lines RAW264.7 (A) and J774A.1 (B) were plated on a 24-well plate at 106 cells/ml and treated for 24 h with the following combination of EdTx subunits (2 µg/ml): 1) PAwt (PA) alone; 2) EF-S414N alone; 3) EF; 4) PA + EF-S414N; 5) PA + EF; 6) PA-U7 + EF-S414N; 7) PA-dFF + EF-S414N. The expression of costimulatory molecules CD80 and CD86 was assessed by flow cytometry. Results are expressed as percentage of positive cells or mean fluorescence intensity and are from four separate experiments. Results are expressed as mean ± 1 SD. Statistical significance: *p ≤ 0.05 when compared with control untreated cells.