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. Author manuscript; available in PMC: 2014 Jun 12.
Published in final edited form as: J Immunol. 2010 Oct 15;185(10):5943–5952. doi: 10.4049/jimmunol.0902795

FIGURE 4.

FIGURE 4

Cytokine mRNA responses to EdTx components and derivatives. Relative cytokine mRNA expression in cervical lymph node cells after 6 h (A) or 24 h (B) of culture in the presence of EdTx components and derivatives. Relative mRNA levels were analyzed by real-time RT-PCR. All the results were normalized against β-actin expression to correct for differences in cDNA concentration of the starting template and are expressed as 20 – (meanCtcytokine – meanCtβ-actin). One-way ANOVA, followed by Duncan’s multiple range test, was used to compare 20-ΔCt values of treated groups (i.e., PA, PA + EF, and mutants of PA + EF) with control untreated. Differences were considered significant at a probability of *p ≤ 0.05.