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. Author manuscript; available in PMC: 2014 Jun 12.
Published in final edited form as: J Immunol. 2010 Oct 15;185(10):5943–5952. doi: 10.4049/jimmunol.0902795

FIGURE 8.

FIGURE 8

F1-V–specific Abs after intranasal immunization with wt PA and EF mutants. A, F1-V–specific serum Ab isotype and IgG subclass responses after intranasal administration of EdTx derivatives. Mice were immunized three times at weekly intervals with 20 µg F1-V only; F1-V plus 2 µg PA plus 2 µg EF; or F1-V plus 2 µg PA-U7 plus 2 µg EF-K346R. The F1-V–specific IgA, IgG, and IgG subclass Ab responses were determined 1 wk after the last immunization. B, F1-V–specific Ab levels in mucosal secretions (saliva and vaginal washes) were assessed 2 wk after the last immunization. The results are expressed as the reciprocal log2 titers ± 1 SD and are from three experiments and five mice/group. Statistical significance: *p ≤ 0.05 when compared with mice immunized with F1-V only.