Table I.

PA and EF derivatives used in these studies

Molecules	Characteristics	References
PA	wt (or native) PA
PA-dFF (E308D)	Mutant with deletion of F314 and F315; internalizes EF, LF to endosomes, but does not translocate them efficiently to the cytosol (E308D has no known functional effect)	43
PA-U7	Mutant lacking the RKKR furin cleavage site, and therefore unable to bind and internalize EF, LF	44
EF	wt (or native) EF
EF-S414N	Mutant with reduced ability to increase cAMP levels in cells	18
EF-K346R	EF mutant devoid of adenylate cyclase catalytic activity due to a point mutation in the ATP binding site	19