Fig. 3.

T-cells labelled with iron nanoparticles show hypointense lesions in the spinal cord of EAE mice that correspond to areas of inflammation and demyelination. (A) and (B) show T₂*-weighted images of the thoracic–lumbar spinal cord in an EAE mouse, in vivo and ex vivo, respectively, where a long hypointense (dark) lesion is visible (white arrows). (C) shows haematoxylin and eosin staining, where inflammation corresponding to the lesion area is seen. (D) shows luxol fast blue staining for myelin with which demyelination is visible as a loss of blue stain.

(Adapted from Anderson et al. (2004).)