Fig. 4.

Lesions detected in the white matter of the lumbar spinal cord of EAE mice using susceptibility-weighted imaging correspond to areas of iron deposition and demyelination. (A) shows the susceptibility-weighted MRI of the lumbar spinal cord of an EAE mouse where a lesion can be seen in the ventral white matter (white arrow). (B) shows DAB-enhanced Perl's staining for the lesion area on which iron deposits can be seen as areas of brown. (C) shows luxol fast blue staining for myelin where demyelination can be seen by the loss of blue stain.

(Adapted from Nathoo et al. (2013a).)