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. 2014 Jun 12;4:5266. doi: 10.1038/srep05266

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Spectral data of previously outlined regions as revealed by image analysis were subjected to statistical analysis of microarray data (SAM). Spectra acquired in positive and negative mode for the GM- and VH-areas (A) were analyzed. (B, C) SAM plots illustrate relative changes of ALS versus control in the respective areas as revealed by unpaired t-statistics. The x-axis illustrates the degree of significance, while the y-axis refers to the observed degree of change. Red illustrates a relative increase in ALS compared to control, while green illustrates a relative decrease in ALS to control. The borders indicate the significance threshold (p < 0.05).