Table 2.
Host plant | Inoculated strain | Nodule numbera | Nodule dry mass (mg) | Plant dry mass (mg) | Plant height (cm) | Chlorophyll content (SPAD unit) |
---|---|---|---|---|---|---|
M. truncatula | KH46c wild-type | 79 | 6.6 | 208 | 12.2 | 44 |
A17 | KH46c ΔvirB6-9 | 38* | 4.3* | 145* | 9.5* | 43 |
M2 wild-type | 102 | 8.4 | 229 | 11.0 | 41 | |
M2 ΔvirB6-9 | 51 | 6.2* | 202 | 11.2 | 44 | |
Uninoculated control | 0 | 0 | 37 | 3.3 | 17 | |
M. truncatula | KH46c wild-type | 35 | 6.1 | 174 | 10.3 | 42 |
F83005-5 | KH46c ΔvirB6-9 | 24 | 5.5 | 158 | 9.8 | 39 |
M2 wild-type | 29 | 4.9 | 156 | 9.5 | 43 | |
M2 ΔvirB6-9 | 22 | 6.7* | 243* | 10.7* | 41 | |
Uninoculated control | 0 | 0 | 44 | 3.3 | 16 | |
M. tricycla | KH46c wild-type | 24 | 12.2 | 315 | 10.5 | 36 |
R108-C3 | KH46c ΔvirB6-9 | 12* | 9.9 | 230 | 10.3 | 34 |
M2 wild-type | 11 | 2.8 | 33 | 4.2 | 19 | |
M2 ΔvirB6-9 | 12 | 3.1 | 33 | 4.2 | 21 | |
Uninoculated control | 0 | 0 | 26 | 3.5 | 16 | |
M. satvia cv | KH46c wild-type | 56 | 1.6 | 95 | 8.5 | 54 |
Agate | KH46c ΔvirB6-9 | 42 | 6.8* | 55 | 7.2 | 45* |
M2 wild-type | 31 | 2.5 | 69 | 13.7 | 31 | |
M2 ΔvirB6-9 | 28 | 2.5 | 85 | 14.6 | 28* | |
Uninoculated control | 0 | 0 | 79 | 12.5 | 21 |
aValues are per plant. The asterisk indicates a significant difference compared with the wild-type strain by t-test (P < 0.05) of three biological replicates.