Table 2.
|
|
|
Average normalized read count per 1 kb exon (delay) |
|
Average normalized read count per 1 kb exon (no delay) |
|
||
---|---|---|---|---|---|---|---|---|
Group | Delay a ( n ) | Delay to R stage b ( n ) | Steady-state mRNA | Polysomal mRNA | No delay ( n ) | Steady-state mRNA | Polysomal mRNA | P -value c |
FIKK kinase family |
8 |
2 |
113 |
25 |
8 |
232 |
30 |
- |
MC-2TM |
7 |
7 |
39 |
18 |
4 |
209 |
72 |
0.0027 |
RESA-like protein |
2 |
1 |
23 |
9 |
2 |
71 |
12 |
- |
Plasmepsin |
5 |
3 |
251 |
40 |
3 |
1,913 |
113 |
- |
Exported proteins |
47 |
31 |
559 |
66 |
71 |
1,234 |
258 |
<0.001 |
ApiAP2 transcription factor |
7 |
1 |
354 |
67 |
11 |
353 |
28 |
- |
Apicoplast-encoded proteins |
11 |
11 |
110 |
97 |
0 |
NA |
NA |
<0.001 |
Apicoplast-targeted proteins |
120 |
50 |
420 |
90 |
255 |
969 |
283 |
- |
Conserved proteins, unknown function |
565 |
237 |
340 |
89 |
1,132 |
514 |
166 |
- |
Totald | 1,280 | 471 | 515 | 114 | 2,727 | 832 | 216 | NA |
aDefined as at least 18 h delay in peak abundance levels in polysomal mRNA as compared to steady-state mRNA.
bSubset of delayed genes, showing peak steady-state mRNA abundance in the trophozoite or schizont stage and peak polysomal mRNA abundance in the ring stage.
cBonferroni-corrected two-tailed Fisher’s exact test for number of genes showing translational delay to the ring stage versus the number of non-delayed genes per group. P-values >0.01 are indicated with a dash.
dTotal number of expressed and cell-cycle regulated genes included in our analysis.
NA., not applicable.