Figure 1.

Phylogenetic relationships of the LD12 SAGs. The phylogenetic tree of the SAGs and the previously sequenced SAR11 genomes was inferred from an alignment of 57 concatenated pan-orthologous proteins. The abbreviations of SAG names from the Integrated Microbial Genomes (IMG) are as in Table S1 in Additional file 1. The subclades to which the SAR11 genomes belong have been defined previously [20] and are indicated with brackets to the right. The letter within parentheses indicates the water systems that the SAR11 cells were isolated from: (a) arctic waters, (c) coastal waters or (o) open ocean waters. All SAGs are from freshwater. Bootstrap support values less than 100% are not shown. The SAGs are colored according to the lake of origin (green = Sparkling; blue = Mendota; red = Damariscotta).