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. 2014 Jan 8;15(1):R12. doi: 10.1186/gb-2014-15-1-r12

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Copyright © 2014 Mukherjee et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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In vivo ZFP36 binding specificity. (A) SNR values for specific motif occurrences within ZFP36 3′ UTR sites relative to the background. (B) Comparison of SNR values for indicated pentamer sequences in ZFP36 sites, all ELAVL1 sites (ELAVL1_all), as well as the highest read count (ELAVL1_best) subset of all ELAVL1 sites. (C) Comparison of SNR values with K and K_d of probe sequences (in different colors) determined using fluorescence anisotropy experiments [48]. The correlation coefficient and equation for the fit of K_d to the reciprocal of the SNR is indicated. (D) Comparison of SNR values (blue) and predicted K_d (red) of nonamer and octamer sequences, as well as half-sites interrupted by one to four uridylates (U) or a non-uridylate (V = A/C/G). SNR, signal-to-noise ratio.