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Diagram illustrating the low coverage of NCS1 exon 1 in 198× Illumina HiSeq shotgun data. The first 72 bases of the first exon of human gene NCS1, including the transcription start site, were uncovered in a 198× whole-genome shotgun data set (#A2). The displayed 2,000 base region is chromosome 9:132,933,910-132,935,910. NCS1 encodes calcium-binding proteins that regulate neurotransmitter release [1].
