Table 4.
Per-lane bias statistics for Illumina HiSeq (Kapa) human NA12878
| Relative coverage | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Data set | GC extremes | Special motifs | |||||||
| Flowcell | # | Lane | Library | GC ≤ 10% | GC ≥ 75% | GC ≥ 85% | (AT)15 | G|C ≥ 80% | Bad promoters |
| C0G7VACXX | 14a | 1 | A | 0.39 | 0.93 | 0.49 | 0.25 | 0.53 | 0.37 |
| 14b | 2 | A | 0.39 | 0.93 | 0.50 | 0.25 | 0.53 | 0.39 | |
| 14c | 3 | B | 0.41 | 0.86 | 0.46 | 0.25 | 0.50 | 0.36 | |
| 14d | 5 | B | 0.41 | 0.85 | 0.45 | 0.26 | 0.50 | 0.36 | |
| 14e | 6 | C | 0.42 | 0.83 | 0.38 | 0.26 | 0.49 | 0.30 | |
| 14f | 7 | C | 0.42 | 0.83 | 0.37 | 0.26 | 0.49 | 0.31 | |
| D0K2WACXX | 14g | 1 | B | 0.41 | 0.89 | 0.55 | 0.25 | 0.85 | 0.38 |
| 14h | 2 | B | 0.40 | 0.89 | 0.56 | 0.25 | 0.85 | 0.39 | |
| 14i | 3 | B | 0.41 | 0.89 | 0.56 | 0.25 | 0.86 | 0.40 | |
| 14j | 4 | A | 0.39 | 0.96 | 0.61 | 0.25 | 0.96 | 0.41 | |
| 14k | 5 | C | 0.42 | 0.85 | 0.43 | 0.26 | 0.70 | 0.32 | |
| 14l | 6 | C | 0.43 | 0.85 | 0.43 | 0.26 | 0.68 | 0.33 | |
| 14m | 7 | C | 0.42 | 0.86 | 0.44 | 0.26 | 0.71 | 0.32 | |
| 14n | 8 | A | 0.39 | 0.97 | 0.62 | 0.25 | 0.95 | 0.41 | |
Bias statistics for the lanes and libraries that compose the HiSeq v3 (low-input Fisher et al. [31] with Kapa reagents) human data set (data set 14 in Table 2). Letters identify the libraries (A = Solexa-77484, B = Solexa-77486, C = Solexa-77483), which were all made using the same protocol.