Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jun 12;5:271. doi: 10.3389/fimmu.2014.00271

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Ha, Pham, Shahsafaei, Naruse, Asano and Thai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Cbx-3^+/− mice fail to mount the high-affinity NP antibody response. (A) ELISA analysis was carried out to measure high-affinity IgG₁ anti-NP4-BSA and total IgG₁ anti-NP25-BSA antibody activity in sera from mice immunized with NP-CGG in alum. Each symbol represents an individual mouse. Bars represent median. Statistical analysis was performed with GraphPad one-way ANOVA. Day 14, n = 9 for each genotype, ***p = 0.006. (B) High-affinity serum IgG₁ anti-NP Ab activity was determined by calculating the ratio of anti-NP4/NP25, *p = 0.02. (C) Serum IgM anti-NP4 and anti-NP25 antibody activity was analyzed by ELISA to NP-BSA as in (A). (D) Plot depicts ratio of IgM anti-NP4 over anti-NP25. (E) Total serum IgG₁ and IgM titers of pre-immune sera from (A) were measured by ELISA.