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. 2014 Jun 12;5:271. doi: 10.3389/fimmu.2014.00271

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Copyright © 2014 Ha, Pham, Shahsafaei, Naruse, Asano and Thai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The T follicular helper cell copulation is reduced in cbx-3^+/− mice. (A) The frequency of spleen TCRβ⁺CD4⁺CXCR5^hiPD-1^hi T_FH population was determined by FACS analysis. Numbers in right bottom corners indicate the percent cells. CXCR5^hiPD-1^hi T_FH cells were gated on the TCRβ⁺CD4⁺ population. (B) Plot represents a compilation of the percent of T_FH population from (A). Each symbol denotes an individual mouse. Bars represent median. Statistical analysis was performed with GraphPad one-way ANOVA. Day 14, n = 8–12 for each genotype, ****p < 0.0001.