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. 2014 Jun 12;5:271. doi: 10.3389/fimmu.2014.00271

Figure 8.

Figure 8

Cbx-3+/− CD8+ T cells directly control the T-dependent immune response. (A) Purified wt or cbx-3+/− spleen and pLN lymphocytes were adoptively transferred into B6-Rag2−/−cγ−/−recipients: group 1 (control, n = 5) received wt B cells, CD4+ and CD8+ T cells; group 2 (experimental, n = 5) received B cells and CD4+ T cells from wt littermate mice and CD8+ T cells from cbx-3+/− mice; group 3 (control, n = 5) received B cells and CD8+ T cells from wt littermate mice and CD4+ T cells from cbx-3+/− mice. (B,C) On day 10 after NP-CGG in alum immunization, spleens of recipients were analyzed by FACS to determine the frequency of GC (from the B220+gate) and TFH (from the TCRβ+CD4+ gate) populations. (D,E) The frequency of CD122+Ly49+CD8+ Treg cells (from the CD3+ gate) was determined by FACS (E) and plotted (D). Numbers on bottom right corners indicate percent cells. Statistical analysis was performed with GraphPad one-way ANOVA; ns, not significant.