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. 2013 Dec 31;14(12):R149. doi: 10.1186/gb-2013-14-12-r149

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Copyright © 2013 Henzler et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of Dlk1-Dio3 miRNAs on reprogramming. (A) Three pools of five downregulated miRNAs located within the Dlk1-Dio3 gene cluster. (B) Quantitative PCR of representative miRNAs from pools 1 to 3 to assess transfection efficiency. (C) Quantitative PCR analysis of Thy1 expression 5 days after OSKM infection and miRNA pool transfection. (D) Relative number of AP + colonies per well 12 days after OSKM infection and miRNA pool transfection. (E) Relative number of Oct4-GFP+ colonies per well 14 days after OSKM infection and miRNA pool transfection. siNTC, si no-template control, *p < 0.05, **p < 0.01, ***p < 0.001, determined by two-tailed Student’s t test.