Figure 2.

NETs have distinct effects on a lacO array inserted into chromosome 13. (a) In a different cell line the lacO array inserted in chromosome 13q tends to be at the periphery: so a NET could promote more peripheral localization or less. (b) Percentage of lacO-tagged 13q loci in each of five erosion shells is plotted with the nuclear periphery (shell 1) to the left in dark blue and more internal localization occurring to the right and with lightening shades of blue. n = 50 cells for each NET. (c) NETs that had strong effects on either cell line and two controls that did not (NET37 and NET55) were retested in two additional experiments to generate standard deviations and P-values. *P < 0.05 and **P < 0.01 comparing the position of the array in the NET-transfected cells to the mRFP control using χ² tests. Effects of individual NETs were highly repeatable with small error bars for standard deviation from the mean and P-values < 0.0001 for NET5, NET29, NET39, NET45 and NET47 in line 5.1. Statistics for each NET are given in Additional file 1. UT, untransfected.