Table 2.
Summary of Gene Ontology terms associated with positive and negative ageCGs affiliated with genes across tissues a
| GO term b | Tissue | Count | P -value | q -value | Fold |
|---|---|---|---|---|---|
|
Positive unique |
|
|
|
|
|
| GO:0030054 - cell junction |
Blood |
42 |
5.2 × 10-10 |
7.0 × 10-7 |
3.1 |
| GO:0048754 - branching morphogenesis of a tube |
Brain |
18 |
1.6 × 10-7 |
3.0 × 10-4 |
5.0 |
| GO:0048878 - chemical homeostasis |
Kidney |
87 |
4.9 × 10-15 |
9.0 × 10-12 |
2.6 |
| GO:0003713 - transcription coactivator activity |
Muscle |
15 |
6.6 × 10-7 |
1.0 × 10-3 |
5.6 |
|
Positive two-way shared |
|
|
|
|
|
| GO:0000902 - cell morphogenesis |
Blood |
37 |
2.3 × 10-14 |
4.0 × 10-11 |
4.9 |
| GO:0003002 - regionalization |
Brain |
41 |
5.3 × 10-15 |
9.6 × 10-12 |
4.6 |
| GO:0000902 - cell morphogenesis |
Kidney |
73 |
2.9 × 10-16 |
6.1 × 10-13 |
3.1 |
| GO:0003712 - transcription cofactor activity |
Muscle |
23 |
3.2 × 10-9 |
4.9 × 10-6 |
4.9 |
|
Positive three-way shared |
|
|
|
|
|
| GO:0007267 - cell-cell signaling |
Blood |
56 |
8.9 × 10-28 |
1.6 × 10-24 |
6.5 |
| GO:0007267 - cell-cell signaling |
Brain |
64 |
4.3 × 10-21 |
7.8 × 10-18 |
4.2 |
| GO:0030182 - neuron differentiation |
Kidney |
117 |
4.5 × 10-37 |
8.2 × 10-34 |
4.1 |
| GO:0016563 - transcription activator activity |
Muscle |
29 |
5.0 × 10-10 |
7.5 × 10-7 |
4.2 |
|
Positive four-way shared |
|
|
|
|
|
| GO:0005886 - plasma membrane |
Blood |
169 |
1.2 × 10-23 |
1.6 × 10-20 |
2.1 |
| GO:0031226 - intrinsic to plasma membrane |
Brain |
119 |
1.7 × 10-36 |
2.5 × 10-33 |
3.8 |
| GO:0031226 - intrinsic to plasma membrane |
Kidney |
209 |
1.3 × 10-60 |
1.9 × 10-57 |
3.7 |
| GO:0006350 - transcription |
Muscle |
102 |
1.5 × 10-18 |
2.6 × 10-15 |
2.6 |
|
Negative unique
c
|
|
|
|
|
|
| GO:0006952 - defense response |
Kidney |
58 |
5.8 × 10-30 |
1.0 × 10-26 |
6.8 |
| GO:0005887 - integral to plasma membrane |
Kidney |
68 |
2.0 × 10-22 |
2.7 × 10-19 |
4.0 |
| GO:0006954 - inflammatory response |
Kidney |
30 |
7.3 × 10-15 |
1.3 × 10-11 |
6.5 |
| GO:0009611 - response to wounding |
Kidney |
39 |
2.2 × 10-14 |
3.8 × 10-11 |
4.6 |
| GO:0005886 - plasma membrane |
Kidney |
138 |
9.1 × 10-14 |
1.2 × 10-10 |
1.8 |
| GO:0006936 - muscle contraction |
Muscle |
15 |
1.5 × 10-11 |
2.5 × 10-8 |
13.0 |
| GO:0003012 - muscle system process |
Muscle |
15 |
7.6 × 10-11 |
1.3 × 10-7 |
11.5 |
| GO:0006941 - striated muscle contraction |
Muscle |
10 |
1.3 × 10-9 |
2.2x10-6 |
21.3 |
| GO:0015629 - actin cytoskeleton |
Muscle |
15 |
3.6 × 10-6 |
4.7 × 10-3 |
4.8 |
| GO:0003009 - skeletal muscle contraction |
Muscle |
5 |
5.0 × 10-6 |
8.3 × 10-3 |
44.3 |
|
Negative two-way shared (kidney and blood only)
d
|
|
|
|
|
|
| GO:0006955 - immune response |
Kidney |
66 |
2.6 × 10-32 |
4.5 × 10-29 |
6.3 |
| GO:0031226 - intrinsic to plasma membrane |
Kidney |
69 |
2.1 × 10-22 |
2.7 × 10-19 |
4.0 |
| GO:0005576 - extracellular region |
Kidney |
105 |
1.8 × 10-15 |
2.3 × 10-12 |
2.2 |
| GO:0044459 - plasma membrane part |
Kidney |
95 |
7.5 × 10-13 |
9.9 × 10-10 |
2.1 |
| GO:0002684 - positive regulation of immune |
|
|
|
|
|
| system process | Kidney | 24 | 1.3 × 10-10 | 2.3 × 10-7 | 5.5 |
aDAVID v6.7 was used with all genes represented on the Methylation27 beadchip as background and gene lists associated for each tissue according to minimum age-associated P-value and positive or negative ageCGs. Enrichment tests were done independently for each tissue. Complete results are shown in supplemental data. All results shown are below q < 0.05 as generated by DAVID analysis [27,28].
bResults were first sorted by shared GO terms among tissues according to whether they overlapped among all four tissues (four-way), among three tissues (three-way), between two tissues (two-way), or unique in only one tissue. GO terms were then sorted by tissue and lowest q-values. The top GO term is shown for each tissue using this sorting scheme.
cThe top five genes affiliated with unique negative ageCGs in each tissue shown here do not include genes from brain because results did not meet q < 0.05for blood tissue see below.
dAll genes affiliated with negative ageCGs in blood were shared with kidney. Results are only shown here for kidney tissue.